VHH Discovery Workflow / Project Plan
Panning Process: Our sdAb library has been applied to the discovery of targets spanning small molecules, proteins, and whole cells. While we have many display methods at our disposal, most panning is performed in a conventional M13 phage display format. By bypassing animal immunization, we’re able to complete panning in 4-6 weeks without the need for a months-long immunization and library creation process.
Phase 1: Sample Submission
Your analyte can be any protein, cell, virus, organic molecule, or inorganic substance that would be expected to be bound by a VHH
Target identity can be named, but this is optional
Submitted sample mass: >100 ug per target, at >10 ug/mL*
Sample medium: phosphate buffered saline (PBS)*
Ship samples to our laboratories in San Diego, CA
*These are IDEAL - please discuss with us if your samples have unique requirements
Phase 2: Library Panning
We immobilize your target on a 96-well polystyrene plate
We perform 3 rounds of panning per target
Additional panning rounds can be run for an additional price
Negative pre-panning to remove unwanted binders is also an available option
Phase 3: ELISA Plate Screening
We select 94 clones + pos/neg controls and test in a 96-well plate
We provide sequencing data for at least 1 and up to 6 confirmed clones
Additional plates / lower affinity clones can be run for an additional price
Custom tags (e.g., fluorophores) and other modifications (e.g., conjugates) are available